Paramagnetic particles coupled with antibodies designed to bind to specific targets are added to the sample.

Detection antibodies – capable of generating fluorescent product – are added.

The objective is to form an immunocomplex consisting of the bead, bound protein, and detection antibody.

At low concentrations, each bead will contain one bound protein, or none.

The sample is loaded into arrays, in the Simoa disc, consisting of more than 200,000 microwells – each large enough to hold one bead.

Enzymatic signal amplification with fluorescent substrate, fluorescence imaging and data reduction.

Data analysis – results can be viewed and analyzed on the board or exported to commonly used software packages or LIMS systems.

96-well plates are spotted at the factory with the target analyte(s) of interest

Diluted samples and standards added to each well and the plates are to create a unique surface chemistry and “vortex” effect

Biotinylated detection antibodies are added to form the other half of the immunocomplex “sandwich”

High sensitivity HRP enzyme-conjugated streptavidin is added in between additional shake cycles.

Finally, a chemiluminescent substrate is added just prior to ultra-sensitive CCD camera imaging, in order to measure the signal intensity produced by each spot.